The RSPCA are appealing to the owners of four horses which were removed from Albert Chaplin Fields in Bridlington.
RSPCA officers were assisted by Humberside Police as they placed the animals into the back of horse boxes at around 1pm today.
Police closed the A164 Bessingby Hill while the operation was underway.
A police spokesman said: "We are acting is support of our colleagues in the RSPCA", but declined to comment further.
An RSPA spokesman for the area added: "Four horses have been removed from this location today (Wednesday 27 January) on veterinary advice because of concerns for their welfare, and are now in RSPCA care.
"The horses were loose and at risk of being hit by a vehicle.
"We want to hear from the owners of the horses, who can call us on 0300 123 8018 and ask to leave a message for RSPCA deputy chief inspector Karen Colman."
It comes after growing calls for action at the site after reports of abandoned horses galloping across Bessingby Hill during busy traffic.